|
KMID : 0603820130190010041
|
|
Journal of Experimental & Biomedical Science
2013 Volume.19 No. 1 p.41 ~ p.47
|
|
|
Construction of 3D Culture Medium with Elastin-like Polypeptide (ELP) Hydrogel for Human Pluripotent Stem Cells
|
|
Lee Jong-hwan
Rhee Ki-Jong
Jung Dong-ju
|
|
|
Abstract
|
|
|
|
Pluripotent stem cells (PSCs) have lots of potential in biomedical sciences owing to its potential to differentiate into any kind of cells in the body. However, it is still a challenge to culture PSCs on a large scale for application to regenerative medicine. Herein, we introduce a synthetic polymer that enables large-scale suspension culture of human PSCs. By employing suspension culture, it became unnecessary to use conventional substrata such as mouse embryonic fibroblast (MEF) or MatrigelTM, which are believed to be main causative sources of xenogeneic contamination in cultured human PSCs in vitro. Human PSCs were cultured in the medium in which elastin-like polypeptide (ELP) dissolved. The ELP in the medium became harden as temperature increases by transforming the medium into a semi-solid gel that supported growth of human PSCs in suspension. Gel-sol transition temperature of ELP can be adjusted by modifying the peptide sequence in which 5 amino acids, Val-Pro-Gly-Xaa-Gly, repeated sequentially. We constructed 3D suspension media having transition temperature around 33~35℃ using an ELP consisted of 40, 60, or 80 repeats of a monomer, which was Val-Pro-Gly-Val-Gly. Among the ELPs, ELP80 was chosen as the best ELP to support growth of human PSCs in suspension culture. This result suggests that the ELP80 can be a medium component for culturing human PSCs in large-scale.
|
|
|
KEYWORD
|
|
|
Pluripotent stem cell, Feeder-free, Elastin-like polypeptide (ELP), Transition
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|